Rna extractions using the plus mini and plus universal kits were. Rneasy protect cell mini kit for rnadna stabilization and. Rneasy mini handbook 062001 5 kit contents rneasy mini kit 20 50 250 catalog no. The quickrna miniprep kit is one of the most innovative rna isolation kits available, designed for the easy, reliable, and rapid isolation of dna free rna from a wide range of cell up to 107 and tissue samples up to 50 mg. Dna, rna, viral nucleic acids and proteins, plus dna and rna cleanup. Rneasy plus micro kit 50 from qiagen sample to insight. Required materials not included with the kit 100% ethanol, acs grade or better to isolate rna from tissue samples, either a rotorstator. Kit contents are stable for up to six months, when properly stored. The rneasy micro kit delivers highly reproducible yields of total rna from small samples. The rneasy minelute cleanup kit should be stored dry at room temperature 1525c and is stable for at least 9 months under these conditions. Rneasy minelute cleanup handbook harvard university.
Rneasy micro kit 50 rneasy plus micro kit 50 mirneasy micro kit 50 rnb300. The mirneasy kit is a separate line from rneasy designed specifically for micrornas, and have their own different tiers ie. For more information, additional and more detailed protocols, and safety. Optimization of the rneasy mini kit to obtain highquality. No claim or representation is intended for their use to identify any specific organism or for a specific clinical use diagnostic, prognostic, therapeutic, or blood banking. Refer to page 23 for details on cleaning the qiavac 24 plus. Timerun, ideal for pcr, northern, dot and slot blotting, array analysis. Storage of rna later reagent at lower temperatures may cause precipitation. Dna free kit from ambion is stored in a 20oc freezer.
Rneasy plus micro kit 50, 74034 from qiagen labsave. C, and should be stored dry and clean at room temperature. For purification of total rna from cultured cells and tissues using gdna eliminator columns. Besides rneasy kits, qiagen rna extraction products cited in the survey include qiazol optimized for fatty tissues, rnase free dnase and allprep dnarna micro kit 59, 60, mirneasy kit 10, 35, 61, oligotex kit 62, 63, mrna isolation. Rneasy plus micro kit 50 from qiagen selectscience. Spin columns, gdna eliminator spin columns, collection tubes, carrier rna, rnasefree water and buffers. Transfer the sample onto an rneasy minelute spin column in a 2 ml collection tube. I isolate tumor cells from breast tumor sections by laser microdissection and extract rna by rneasy plus micro kit qiagen. Rneasy mini handbook 042006 7 the rneasy mini kit and rneasy protect mini kit are intended as generalpurpose devices. For purification of up to 100ug total rna from animal cells or tissues, yeast, or bacteria. Oragenerna purification protocol using the qiagen rneasy. For small number of cells homogenisation can be done.
Preparations per kit 20 50 250 rneasy mini spin columns pink 20 50 250 collection tubes 1. Qiagen rneasy plus mini kit, 50 preps, 30mg sample, 30 to 50l elution volume, tissue, cells sample, total rna purification, silica technology, spin column format, 25 min. Cross reference of genelute to qiagen nucleic acid. This post contains a slightly modified rneasy kit protocol. Total rna can reliably be purified from small numbers of cells, including a single cell, as well as from small amounts of standard tissues see figures reliable rna isolation from a single cell, highly reproducible yields for sensitive applications and highquality total rna from fine needle aspirates. Rnase free dnase set box, containing rnase free dnase, buffer rdd, and rnase free water, should be stored immediately upon receipt at 28c. Product use limitations the qiavac 24 plus is designed for vacuum processing of qiagen spin columns in parallel. The mirneasy mini kit enables lowthroughput mirna and total rna purification using spin columns. The rneasy plus micro kit is specially designed for limited amounts of. A short workflow enables rna isolation with genomic dna removal in less than 25 minutes see flowchart rneasy plus procedure. For phenolfree total rna extraction from cellstissues with removal of genomic. The rneasy micro kit is shipped at ambient temperature. Rneasy plus mini kit vs quickrna miniprep 50 preps.
The procedure combines a unique buffer system with zymospin column technology to yield hig. For stabilization and purification of up to 100 g total rna from tissues. Prepare 70% and 80% ethanol solution using rnase free water. The supernatant samples 1ml each will be transfered.
All contents of the purelink rna mini kit are shipped at upon receipt, store all contents at room temperature. For purification of total rna from small samples, including animal and human cells. Genelute total rna purification kit 100 genelute total rna purification maxi kit 8 from small samples. Qiagen rneasy plus mini kit, 250 preps, 30mg sample, 30 to 50l elution volume, tissue, cells sample, total rna purification, silica technology, spin column format, 25 min. The rneasy plus micro kit integrates fast, convenient purification of up to 45 g rna with. Total rna and mirna can also be copurified from formalinfixed, paraffinembedded. Rneasy protect mini kit 50 from qiagen selectscience. Purelink rna mini kit 12183018a 50 preps 12183025 250 preps shipping and storage room temperature. Rneasy kits discussed below in more detail is the most commonly cited rna extraction method. Then, 800 l rnaprotect bacteria reagent qiagen, usa was added and the contents in the tube were vortexed prior to a 5 min incubation at room temperature rt. Qiagens rneasy mini kit and rneasy plus micro kit having low yields of rna mar252009 i tried using the rneasy plus micro kit claims it can isolate rna from a single cell at first to extract rna from 20,000 cells human es cells but i only got a very low yield of 22 ngul 14 ul elution volume. Rna isolation with trizol invitrogen and qiagen rnaeasy. Print bookmark share for purification of total rna from small samples, including. A wide range of kits deliver manual or automated purification of highquality.
Qiagen rneasy plus micro kit, 50 preps, 14l elution volume, 5mg tissue amount, tissue, cells sample, total rna purification, spin column format, silica technology, ideal for northern, dot and slot blotting, endpoint rtpcr, quantitative, realtime rt. The kit includes rnase free lysis and wash solutions that protect rna from rnases while liberating the. Rna free from phenol contamination are achieved using the combination of qiazol lysis. Are qiagen gdna eliminator columns compatibile with trizol. The quickrna miniprep plus kit is one of the most innovative rna isolation kits available, designed for the easy, reliable, and rapid isolation of dna free rna from cells, all tissue types, whole blood, and biological fluids. Rneasy plus micro kit protocol using rna carrier and. The following brief version of the protocol is provided for your convenience. Rneasy fibrous tissue mini kit protocolrna quantification. Purification of highquality rna from a small number of. For phenol free total rna extraction from cellstissues with removal of genomic dna contamination. Click on the product or protocol links to be directed to the manufacturers pages. Rneasy mini kit and phenol free total rna purification kit the cell pellet was reconstituted with 400 l of sterile minimal media.
Preprotocol using latex or nitrile powder free gloves wipe work surface and all. With the exception of embryos, each kit generally extracted. No claim or representation is intended for its use to identify any specific organism or for clinical use. With regard to rna quality, the rins of rna samples isolated via the plus mini and maxwell 16 kits were consistently higher than those of samples extracted via the remaining three kits and for all tissues, these kits produced intact rna with average rin values. Are the rneasy mini spin columns same in rneasy plus mini kit. The rneasy micro kit is shipped at room temperature 1525c. Quickstartprotocol rneasy plus mini kit january 2011 igem 2016. But i would like to also get dna from the same microdissected samples. Ensure to work in an rnase free environment by cleaning workspace and pipettes with a rnase decontamination product and use filter tips.
The rneasy mini kit, rneasy protect mini kit including rna later rna stabilization reagent, and rneasy plant mini kit should be stored dry at room temperature 1525c and are stable for at least 9 months under these conditions. The rneasy plus mini kit, which is designed for rna purification from animal. Total rna isolation with rneasy micro qiagen spin protocol. A comparison of commerciallyavailable automated and manual. Treatment of free living or sessile bacterial cells with acetoneethanol 1. Discard the flowthrough liquid and place the column in a new clean 2 ml tube.
Qiavac 24 plus handbook 102010 5 storage the qiavac 24 plus is shipped at room temperature 1525. Total rna isolation with rneasy micro qiagen karine serre. The purelink rna mini kit is a columnbased kit used to isolate highquality total rna from a wide variety of sample types in 20 minutes using standard laboratory equipment. Kit components and storage reagents are provided for isolating rna from 50 samples. For lasermicrodissected formalinfixed, paraffinembedded ffpe tissues, the qiaamp dna ffpe tissue kit cat. Rna isolation protocol using the qiagen rneasy mini kit. Qiagen rneasy plus micro kit, 50 preps, 14l elution volume, 5mg tissue amount, tissue, cells sample, total rna purification, spin column format, silica technology, ideal for northern, dot and slot blotting, endpoint rtpcr, quantitative, realtime. I want to use the qiagen rneasy micro plus kit on 4 crab hemolymph pellet samples and the four corresponding supernatant samples github issue 577. Rneasy plus micro and mini kits qiagen online shop.
Rneasy plus micro kit protocol using rna carrier and supernatant. Kit contents the components included in the purelink rna mini kit. The rneasy plus micro kit purifies total rna from up to 5 x 10 5 cells or 5 mg tissue, and the rneasy plus mini kit isolates total rna from up to10 7 cells or 30 mg tissue. Plasmid dna purification genomic dna purification cat. The mirneasy micro kit is designed for purification from small amounts of cultured cells, sorted cells, laser capture microdissection samples from cryosections, and various animal and human tissues. Isolation and rna extraction of neurons, macrophages and. Product use limitations the rneasy minelute cleanup kit is intended as a generalpurpose device. The goal is to see if there is any rna in the supernatant samples and if that can help increase the rna yields for each crab sample. For phenolfree total rna extraction from cellstissues with oncolumn gdna removal. Rneasy fibrous tissue mini kit protocolrna quantification tools rneasy fibrous tissue kit, rnase zap wipes, liquid nitrogen, ceramic mortar and pestle, tube holder, ul and 200ul pipettes with tips, scissors, tweezers, face mask for at least steps 2 and 3. Nucleic acids within urinary exosomesmicrovesicles are. For highthroughput purification in a 96well format, the mirneasy 96 kit is available.
Rneasy midi kit 75142 rneasy maxi kit 75162 animal cells and tissues, with gdna eliminator spin columns rneasy plus mini kit 744 fiberrich tissues 100 ug rna rneasy fibrous tissue mini kit 74704 1 mg rna rneasy fibrous tissue midi kit 75742 fatty tissues 100 ug rna 1 mg rna rneasy lipid tissue mini kit 74804. The kit includes rnase free lysis and wash solutions that protect rna from rnases while liberating the rna from dna, proteins, and other cellular debris. Nov 23, 2015 sheared wholecell lysis coupled to the rneasy mini kit. Rna amounts from 45 g down to a few picograms, which correspond to less than 1 cell, can be purified from enzymatic reactions e. Rneasy plus mini kit vs quickrna miniprep 50 preps this page will help you compare kit specs and protocols across manufacturers. Add 3050 l of rnase free water onto the membrane of the column 20. The remaining components of the rneasy micro kit should be stored dry at room temperature. Rneasy plus micro kit using the mirna extraction method, blue microvesicle rna isolated with rneasy plus micro kit using the mirna extraction method. Rneasy minelute cleanup handbook 102010 7 introduction the rneasy minelute cleanup kit uses novel technology to purify and concentrate rna. Rneasy plus mini kit 250 from qiagen sample to insight.
725 631 305 602 722 1266 836 98 1426 1452 617 822 1449 343 160 456 508 828 552 178 1274 366 1416 888 659 226 257 919 933 821 1393 901 1252 1059 884 373