The michigan cancer foundation is now known as the barbara ann karmanos cancer institute prior to mcf7, it was not possible for cancer. While matrixbased culture models allow for the growth of cell lines in 3. When compared to mcf7 cells cultured in 2d monolayer culture, mcf7. The major requirement of a cell culture laboratory is the need. The human breast adenocarcinoma cell line mcf7 michigan cancer foundation7 has served for over 40 years as a standard model for in. A stable cell line mcf7 derived by pleural effusion from a breast cancer patient has been demonstrated to contain significant amounts of 17fiestradiol receptor. Downmodulation of hla class i expression by dexamethasone in mcf7 cell line article pdf available in journal of physiology and biochemistry 534. Cells should be subcultured while still in the exponential phase.
Transfer the cell suspension to the centrifuge tube with the medium and cells from step 1, and centrifuge at approximately 125 xg for 5 to 10 minutes. Despite its origin from the metastases of an advanced tumor, the cell line is noninvasive and represents a model of. Mcf7 breast cancer cell line, a model for the study of th e association between inflammation and abcg2mediated multi drug resistance 347 mucosa chan et al. Mcf7 breast cancer cell line, a model for the study of. Choosing the right cell line for breast cancer research breast. Cells may stop growing when the ph is between ph 7. Mcf7 is a human breast cancer cell line that was first isolated in 1970 from the malignant. Mcf7 cells are fairly large adherent cells, with typical cell size measuring 2025 microns. Mcf7 is the acronym of michigan cancer foundation7, referring to. Genetic variability in a frozen batch of mcf7 cells invisible in. Cells that are difficult to detach may be placed at 37c to facilitate dispersal. Mcf7 was derived from the pleural effusion from a 69 year old female suffering from a breast adenocarcinoma1. In most cases, cultures at a high cell density exhaust the medium faster than those at low cell density as is evident from the change in ph. Mcf7 human breast cancer cells form differentiated microtissues.
A drop in ph is usually accompanied by an increase in cell density, which is an indicator to subculture the cells. It was named after the michigan cancer foundation mcf and is the most studied human breast cancer cell line in the world2. The popularity of mcf7 is largely due to its exquisite hormone sensitivity through expression of oestrogen receptor er, making it an ideal. Mcf7 breast adenocarcinoma national cancer institute. Mcf7 is a commonly used breast cancer cell line, that has. The recommended medium is emem containing 2 mm lglutamine, 0. Mcf7 is a breast cancer cell line isolated in 1970 from a 69yearold caucasian woman. Mcf7 cells human breast adenocarcinoma cell line cell. The mcf7 cell line, which was established from a pleural effusion at the michigan cancer foundation in 1973, is by far the most commonly used xenograft model of breast cancer. Mcf7 is the acronym of michigan cancer foundation7, referring to the institute in detroit where the cell line was established in 1973 by herbert soule and coworkers.
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